Effective clinical management of small cell lung cancer (SCLC) requires sensitive and matrix-tolerant quantification of pro-gastrin-releasing peptide (ProGRP). Herein, we report a nanoarchitectured electrochemiluminescence (ECL) sandwich immunosensor that combines a three-dimensional reduced graphene oxide/gold (3D-rGO@Au) capture interface with a luminescent europium metal-organic framework (Eu-MOF) signal probe labeled with Ab2 for ProGRP measurement in serum and pleural effusion. The Eu-MOF formed uniform octahedral particles and provided efficient antenna-effect-assisted ECL emission with K₂S₂O₈ as the coreactant, while the 3D-rGO@Au interface increased antibody loading and accelerated interfacial electron transfer. The assay achieved a detection limit of 0.035 pg mL⁻¹ over a broad linear range, showed satisfactory recovery in two clinical matrices, and agreed well with commercial ECLIA measurements. Beyond combining two previously reported material families, the main contribution of this work is the clinically oriented integration of a high-loading conductive interface, a Eu-MOF luminophore probe, mechanistic ECL/CV/EIS validation, and real-sample verification for ProGRP detection.
Luo et al. (Sun,) studied this question.