Multifunctional switch mediates the catalytic activity and product ratio in fungal CYP512W2

Key Points

• The research aims to understand how specific sites in CYP512W2 influence its catalytic behavior and the resulting product outcomes.
• Characterization of the CYP512W2 enzyme
• Site-directed mutagenesis of amino acids I108 and I364
• Analysis of changes in catalytic activity and product ratios
• Mutations I108N and I364H reversed the product selectivity
• Mutations I108S and I364C increased total production of products