Biopsy-derived colonoid air-liquid interface monolayers reveal persistent mucosal defects in ulcerative colitis patients

Key Points

• Colonoid monolayers derived from ulcerative colitis patients reveal significant mucosal defects under non-inflammatory conditions, indicating ongoing issues.
• These defects in developmental and functional aspects suggest that even without inflammation, healing problems persist in ulcerative colitis.
• Assessment through culturing of biopsies into air-liquid interface colonoid monolayers is utilized to study mucosal development, healing, and function.
• Findings highlight that defects in the mucus barrier function contribute to chronic challenges in managing ulcerative colitis.

Abstract