Chemical proteomics-based identification of artesunate as a POLH-PHB1 interaction disruptor

Key Points

• The study aims to identify interactions disrupted by artesunate targeting POLH in human kidney cells.
• Conducted a chemical proteomics approach to profile POLH interactome changes.
• Utilized HEK293T human embryonic kidney cells for experimentation.
• Analyzed the inhibitory effects of artesunate on protein interactions.
• Identified artesunate as a disruptor of POLH-PHB1 interactions.
• Demonstrated significant alterations in the POLH interactome upon treatment with artesunate.