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开发了一种高通量系统以并行监测多个基因的表达。通过高速机器人打印互补DNAs于玻璃上的微阵列,用于对应基因的定量表达测量。由于阵列的小型格式与高密度,可采用2微升的杂交体积,从2微克总细胞信使RNA中衍生的探针混合物中实现罕见转录本的检测。通过同时进行的双色荧光杂交,对45个拟南芥基因进行了差异表达测量。
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Mark Schena
Dari Shalon
Ronald W. Davis
Science
Stanford University
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Schena 等人(周五,)研究了该问题。
www.synapsesocial.com/papers/69dbd2bff7e0c66ced83666f — DOI: https://doi.org/10.1126/science.270.5235.467
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