July 1, 1989Open Access

通过毛细管气相色谱法同时测定厌氧菌挥发性和非挥发性酸性发酵产物

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Abstract

一种之前用于青贮饲料中有机酸分析的方法被应用于检测和定量瘤胃细菌及厌氧真菌纯培养物中的酸性发酵产物（C1至C6挥发性脂肪酸、乳酸和丁二酸）。在分离前，这些酸被转化为叔丁基二甲基硅烷衍生物，使用长30米的DB1毛细管气相色谱柱进行分离。甲酸和丁二酸的定量回收率被发现与原研究中报道的其他酸回收率相当。乳酸的定量回收率被发现取决于衍生化后样品在室温下至少储存24小时。该方法的一大特点是能够同时测定范围广泛的挥发性和非挥发性酸性产物。

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Authors

Anthony J. Richardson

A. G. Calder

C.S. Stewart

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Letters in Applied Microbiology

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通过毛细管气相色谱法同时测定厌氧菌挥发性和非挥发性酸性发酵产物

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