PulseExploreJournal ClubDebatesTrendingResearchersJournals
Instagram
HomeExploreJournal ClubTrending
Synapse
⌘+K
Synapse
August 6, 2015PLoS ONE97 citationsOpen Access

Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing

View Full Paper
CLChrysanthia A. LeontiouCyprus Institute of Neurology and GeneticsMHMichael D. HadjidanielChildren's Hospital of Los AngelesPMPetros MinaMedicover Genetics (Cyprus)

Key Points

Key points are not available for this paper at this time.

Abstract

Our findings indicate that the MethylEdge Bisulfite Conversion System (Promega) was shown to have the best performance among the kits. In addition, the methylation level of two of our DMRs, EP6 and EP10, was confirmed. Finally, we showed that bisulfite amplicon sequencing is a suitable approach for methylation analysis of targeted regions.

Ask AI
Helpful
Bookmark
Share
View Full Paper

Cite This Study

Leontiou et al. (2015) studied this question.

synapsesocial.com/papers/692f0eaf74f629f97ff58095https://doi.org/10.1371/journal.pone.0135058
Ask AI
Helpful
Bookmark
Share
View Full Paper

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1PCR Quantitation of Fetal Cells in Maternal Blood in Normal and Aneuploid Pregnancies1997 · 367 citations
  2. 2Invasive Prenatal Diagnostic Procedures 20052005 · 78 citations
  3. 3A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.1992 · 3,290 citations
  4. 4High resolution profiling of human exon methylation by liquid hybridization capture-based bisulfite sequencing2011 · 27 citations
  5. 5Evaluation of molecular tests for prenatal diagnosis of chromosome abnormalities2003 · 76 citations