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May 6, 2026Annals of Work Exposures and Health

71 In vitro human alveolar macrophage (ImmuPHAGE™) responses to particulate matter exposure

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Authors

EZElinda ZeqiriEHEwelina HoffmanDVDriton Vllasaliu

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Overview

Randomized trial evaluates respiratory toxicity of particulate matter in alveolar macrophages, suggesting health impacts of air pollution.

Key Points

  • This study aims to evaluate the in vitro respiratory toxicity of particulate matter in human alveolar macrophages.
  • Exposed human alveolar macrophages (ImmuPHAGE™) to PM2.5 concentrations (25 to 100 µg/mL) for 24 and 48 hours.
  • Assessed cell viability, cytotoxicity, and phagocytic function using standard assays and fluorescent microspheres.
  • Utilized high-content imaging for single-cell profiling of morphology and stress markers.
  • Significant reductions in mitochondrial activity across all exposure levels (P < 0.05).
  • Phagocytic activity decreased after 24 hours (P < 0.05) but increased after 48 hours (P < 0.05).
  • Distinct phenotypic changes observed: early responses featured elevated stress, while later responses showed enlarged cells and increased vacuolation.

Cite This Study

Zeqiri et al. (2026) studied this question.

synapsesocial.com/papers/69fadaab03f892aec9b1e670https://doi.org/10.1093/annweh/wxag024.103
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