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April 13, 2026Naunyn-Schmiedeberg s Archives of PharmacologyOpen Access

The striking divergence of ABCB1 mRNA expression and P-glycoprotein protein levels in M1 and M2 macrophages associates with the microRNA miR-21-3p, a regulator of “RNA binding protein, mRNA processing factor”

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Authors

KHKatharina HamburgJWJohanna WeissJSJulia Carolin Stingl

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Overview

Compares drug transporter expression in M1 and M2 macrophages, revealing important regulatory interactions.

Key Points

  • The research aims to investigate the differences in drug transporter expression between M1 and M2 macrophages and how microRNAs regulate these differences.
  • Differentiation of THP-1 monocytes into M1 and M2 macrophages
  • Quantitative polymerase-chain reaction for evaluating 84 drug transporter mRNA levels
  • Western blot for assessing P-glycoprotein protein levels
  • Evaluation of 46 microRNA species for regulatory roles
  • Seven transporter genes were lower expressed in M1 compared to M2 macrophages, including ABCG2 and SLCO2B1
  • Twenty-one transporter genes were higher expressed in M1 macrophages
  • ABCB1 mRNA was up-regulated threefold in M1, but P-gp protein levels were 2.4-fold lower
  • Increased expression of miR-21-3p was associated with discrepancies in ABCB1 expression

Cite This Study

Hamburg et al. (2026) studied this question.

synapsesocial.com/papers/69dc88d83afacbeac03ea954https://doi.org/10.1007/s00210-026-05288-8
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells2026
  2. 2Modulation of Macrophage Inflammatory Responses by UDP-Glucuronosyltransferase-Mediated PGE2 Glucuronidation2026
  3. 3The Differential Effect of a Shortage of Thyroid Hormone Compared with Knockout of Thyroid Hormone Transporters Mct8 and Mct10 on Murine Macrophage Polarization2024 · 3 citations
  4. 4Changes in the spectrum of extracellular vesicles produced by THP-1 cells during polarization toward M1 or M2 macrophages2025
  5. 5Identification of novel protein biomarkers of macrophage polarization using comparative proteomic analyses of murine primary macrophages2025 · 4 citations