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The normalization strategy presented here is a prerequisite for accurate RT-PCR expression profiling, which, among other things, opens up the possibility of studying the biological relevance of small expression differences.
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Jo Vandesompele
Katleen De Preter
Filip Pattyn
Genome biology
Ghent University Hospital
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Vandesompele et al. (Tue,) studied this question.
www.synapsesocial.com/papers/69d6b58bf174babf6cab3377 — DOI: https://doi.org/10.1186/gb-2002-3-7-research0034
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