Winnie mice are a widely used in vivo model of inflammatory bowel disease and carry a missense mutation in the Muc2 gene. Here, we present a protocol for genotyping Winnie mice using TaqMan allelic discrimination quantitative PCR. We describe tissue collection, rapid crude DNA extraction, probe-based amplification with dual-labeled fluorophores, and fluorescence-based genotype calling in a single reaction. This protocol enables qualitative SNP genotyping without post-amplification processing and can be readily adapted to other defined point mutations.
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Mansoori et al. (Sat,) studied this question.
synapsesocial.com/papers/69df2a4be4eeef8a2a6af75a — DOI: https://doi.org/10.1016/j.xpro.2026.104497
Behzad Mansoori
The Wistar Institute
Chengyu Liang
STAR Protocols
The Wistar Institute
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