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April 18, 2026Open Forum Infectious DiseasesOpen Access

Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion Abilities

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Authors

MIMasahiro ItoMKMisaki KataokaMAMio Aso

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Overview

Reveals that specific Lactobacillus crispatus strains enhance antimicrobial peptide production in vaginal epithelial cells, suggesting potential health benefits.

Key Points

  • This study aims to understand how specific strains of Lactobacillus crispatus influence the secretion of human β-defensin-2 in vaginal epithelial cells.
  • Co-incubated vaginal epithelial cells with live or heat-killed Lactobacillus strains.
  • Quantified mRNA levels of HβD-2, IL8, TNFα, and MUC1 using quantitative PCR.
  • Measured HβD-2 protein levels using ELISA.
  • Stained adherent Lactobacillus cells and counted using fluorescence microscopy.
  • HMS-115 and HMS-122 strains significantly increased HβD-2 mRNA expression in VK2/E6E7 cells.
  • Eighteen other strains showed comparable HβD-2 mRNA levels to control groups.
  • HMS-115 promoted HβD-2 expression and maintained its adherence ability even when heat-killed.
  • Incubation with live HMS-115 induced pro-inflammatory cytokine expression; heat-killed did not.

Cite This Study

Ito et al. (2026) studied this question.

synapsesocial.com/papers/69e31fcb40886becb653efb1https://doi.org/10.1093/ofid/ofag193
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Lactobacillus crispatus CCFM1339 Inhibits Vaginal Epithelial Barrier Injury Induced by Gardnerella vaginalis in Mice2024 · 25 citations
  2. 2Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract.2024 · 5 citations
  3. 3Exopolysaccharides of Lactobacillus crispatus mediate key balancing interactions with the vaginal mucosa2026
  4. 4Bacillus subtilis Induces Human Beta Defensin-2 Through its Lipoproteins in Human Intestinal Epithelial Cells2024 · 2 citations
  5. 5Insights into host dependency from a chemically defined medium for the human vaginal bacterium Lactobacillus crispatus2025