The aim of this study was to investigate the effect of phyto-additive mixture supplementation on semen quality and on some reproductive parameters after artificial insemination in rabbits. The trial run 120 days on 20 adult New Zealand white rabbit bucks that were allocated into two different groups, first was control (CON; n = 10) fed with commercial pelleted-feed and second was considered experimental group (EXP; n = 10) which received in feed a natural feed additive mixture (0.1% of dried Chlorella vulgaris powder and 0.1% of dried Laurus nobilis leaves powder). Consequently, the quality assessment of semen by the Computer Assisted Semen Analyzer (CASA) system, samples were instrumentally inseminated on rabbit does for two consecutive reproductive cycles, and productive and reproductive indexes were evaluated. Results demonstrate that while spermatozoa concentration and ejaculate volume did not differ significantly among experimental groups or between reproduction cycles, spermatozoa motility parameters were significantly enhanced in rabbits receiving the phyto-additive mixture, as evidenced by increased total motility (87.83% vs. 70.63%) and progressive motility (75.68% vs. 50.10%) compared with the control group (p < 0.01). No differences were observed in prolificacy traits during the first reproductive cycle, whereas in the second cycle the phyto-additive treatment increased the number of kits born alive per litter (12.29 vs. 10.19; p < 0.05) and improved kit growth performance at birth (79.17 vs. 66.75 g), at weaning (1085.28 vs. 963.15 g), and in average daily gain (28.75 vs. 25.61 g/day). The study provides evidence of alternative practises based on feeding programme to enhance reproductive traits in rabbit production. The goal is to provide farmers with examples of good farming practise (such as precision farming), focused on sustainability and efficiency, and a certain transfer of knowledge.
Vizzarri et al. (2026) studied this question.