Analytical challenges for mapping non-canonical and non-protein ubiquitin/Ubl modifications by mass spectrometry

Key Points

• Mapping non-canonical ubiquitin modifications reveals complex cellular processes.
• Details about ubiquitin's diverse roles remain largely unexplored, emphasizing a need for advanced techniques.
• Challenge of mapping using mass spectrometry focuses on detecting various modifications not typically associated with ubiquitin.
• Findings suggest a deeper understanding of cellular mechanisms may emerge, implying a need for innovation in analytical methods.