The aim is to simultaneously profile chromatin accessibility and DNA-associated protein binding in single cells.
Utilized the BGI C4 platform for data capture
Performed single-cell ATAC-seq on K562 cell line
Conducted single-cell CUT&Tag on liver cancer samples
Successfully captured chromatin accessibility and binding data in single cells
Identified specific protein interactions in K562 and liver cancer cells
Abstract
This project is based on the BGI C4 platform to simultaneously capture single-cell ATAC-seq data and single-cell CUT&Tag data of K562 cell line and a liver cancer sample.