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The study aims to develop a sterilization protocol for in vitro culture of hybrid mandarin seeds and induce nucellar seedling growth.

March 18, 2026Вестник российской сельскохозяйственной науки / Vestnik of the Russian Agricultural Science0 citations

Introduction to Sterile Culture of Hybrid Mandarin Seeds from Distant Crosses

Introduction to Sterile Culture of Hybrid Seeds From Distant Crosses of BLAN.VAR

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Breeding research demonstrates high germination rates of nucellar seedlings in hybrid mandarin seeds, implying effective culture protocols.

The study aims to develop a sterilization protocol for in vitro culture of hybrid mandarin seeds and induce nucellar seedling growth.
Developed a sterilization protocol using Domestos gel, 96% ethyl alcohol, and fruit burning.
Introduced seeds from seven distant cross combinations into sterile culture.
Utilized the Murashige and Skoog medium enhanced with 6-BAP and mesoinositol for germination.
Achieved 96% yield of sterile culture with low contamination rates (4.0 to 16.7%).
Seed germination rates ranged from 63.1% to 87.2% across different combinations.
Highest germination noted in the combination 98-9 × pollen mixture at 87.2%.

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Gvasaliya et al. (2025) studied this question.

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March 18, 2026Вестник российской сельскохозяйственной науки / Vestnik of the Russian Agricultural Science0 citations

Introduction to Sterile Culture of Hybrid Seeds From Distant Crosses of BLAN.VAR

Authors

Breeding research demonstrates high germination rates of nucellar seedlings in hybrid mandarin seeds, implying effective culture protocols.

The study aims to develop a sterilization protocol for in vitro culture of hybrid mandarin seeds and induce nucellar seedling growth.
Developed a sterilization protocol using Domestos gel, 96% ethyl alcohol, and fruit burning.
Introduced seeds from seven distant cross combinations into sterile culture.
Utilized the Murashige and Skoog medium enhanced with 6-BAP and mesoinositol for germination.
Achieved 96% yield of sterile culture with low contamination rates (4.0 to 16.7%).
Seed germination rates ranged from 63.1% to 87.2% across different combinations.
Highest germination noted in the combination 98-9 × pollen mixture at 87.2%.

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Gvasaliya et al. (2025) studied this question.

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