Background: Conditioning activity (CA) is used to elicit post-activation performance enhancement (PAPE), but it is unclear whether load response principles from back squat models generalize to unilateral split squat conditioning when external load and surface instability are manipulated together. Thus, the current study examined acute effects of stable vs. unstable split squat CA with or without external load on jump performance and phase-specific electromyography (EMG). Methods: Twenty men completed a randomized crossover of three CAs (2 × 3 reps): unloaded stable split squat (SS), unloaded BOSU SS, and BOSU loaded at 50% split squat one-repetition maximum. Single leg jump (SLJ) and countermovement jump (CMJ) were assessed pre-CA and at 3 min (SLJ) and 4 min (CMJ) post-CA. EMG was recorded from the biceps femoris (BF), semitendinosus (ST), vastus lateralis (VL), vastus medialis (VM) gluteus medius (Gmed), peroneus longus (PL), gastrocnemius lateralis (GL) and gastrocnemius medialis (GM). Signals were time-normalized across the split squat cycle and quantified using phase-specific area under the curve (AUC) (descending/ascending). Results: SLJ and CMJ increased after all conditions compared with the pre-test (p < 0.05). SS and unloaded BOSU SS produced comparable jump outcomes, whereas BOSU loaded yielded the greatest CMJ increase (p < 0.04). Unloaded BOSU SS selectively increased hamstring activation (BF, ST) without changes in Gmed or PL. BOSU loaded increased EMG amplitude across all measured muscles. Conclusions: External load primarily drives acute CMJ potentiation, whereas instability mainly redistributes recruitment toward the hamstrings without improving jump performance beyond the stable condition. These findings indicated that when the goal is acute jump enhancement, external load should be prioritized, whereas unstable surfaces may be used to selectively target posterior chain activation.
Sim et al. (2026) studied this question.