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May 8, 20260 citationsOpen Access

Single-Molecule, Real-Time Immunoassay for Protein-Protein Interactions

Development of a single-molecule, real-time, label-free, mass-resolved immunoassay

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Authors

CBCarraugh Brouwer

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Overview

Randomized trial reveals quantitative protein-protein interactions in complex samples, indicating potential for improved biomarker detection.

Key Points

  • This research aims to develop a label-free, real-time immunoassay to directly observe protein-protein interactions.
  • Developed an immunoassay based on interferometric scattering microscopy (iSCAT) for protein detection.
  • Utilized IgM as a model system to measure binding events in human serum.
  • Quantified concentrations of IgM binding events and compared with bulk measurements using ELISA.
  • Demonstrated real-time detection of individual IgM binding events with linear association rates over three orders of magnitude.
  • Observed strong correlation between detected concentrations and ELISA measurements, validating the method.
  • Achieved single-molecule sensitivity and molecular-weight discrimination in complex biological samples.

Cite This Study

Carraugh Brouwer (2026) studied this question.

synapsesocial.com/papers/69fd7fb8bfa21ec5bbf083edhttps://doi.org/10.14288/1.0452388
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