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April 13, 2026Scientific Reports0 citationsOpen Access

Genome Concatenation for Accurate Dual RNA-seq Mapping in Fungal Cocultures

Genome concatenation enables accurate dual RNA-seq mapping for lignocellulolytic fungi in coculture

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Authors

BSB. P. SolleroRCRutiane Moreira de Jesus CostaRTRoberto Coiti Togawa

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Overview

Dual RNA-seq reveals efficient transcriptome mapping methods in fungal cocultures, suggesting improved accuracy for gene expression studies.

Key Points

  • Evaluate the effectiveness of two mapping strategies for dual RNA-seq in fungal coculture systems.
  • Utilized dual RNA-seq for transcriptome analysis of Trichoderma reesei and Pleurotus citrinopileatus.
  • Applied sequential alignment against individual genomes and a concatenated reference genome.
  • Employed Illumina NovaSeq sequencing to generate 175 million reads.
  • Achieved mapping rates exceeding 96% for both strategies.
  • No significant differences found between mapping strategies or genome order.
  • Concatenated mapping reduced computational time by approximately 93%.
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Cite This Study

Sollero et al. (2026) studied this question.

synapsesocial.com/papers/69dc88d83afacbeac03eaa1ahttps://doi.org/10.1038/s41598-026-43548-y
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