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May 6, 2026Circulation

Proteomic analysis links 14 proteins to ischemic stroke risk in women via metabolic and inflammatory pathways.

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Why the study?

Ischemic stroke heavily affects women, yet reliable biomarkers predicting risk remain limited, hindering early detection and prevention.

Are specific plasma proteomic profiles and pathways associated with incident ischemic stroke risk in women?

Population

450 incident IS cases and 450 matched controls among women in the Nurses' Health Study

Comparison

Incident IS cases vs matched controls

Design

Nested case-control study

Follow-up

Median time-to-event of 5.5 yrs

Key result

Proteomic analysis of 5,440 proteins identified 14 proteins significantly associated with ischemic stroke risk in women (q<0.05), highlighting distinct metabolic and inflammatory pathways.

Authors

KSKevin SanchezBABenjamin AbijahDHDanielle Haslam

Discussion

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Overview

Hypothesis-generating for proteomic IS biomarkers in women; leaves open clinical utility pending prospective validation.

Key Points

  • This study aims to identify protein biomarkers and their associated pathways related to ischemic stroke risk in women.
  • Nested case-control study with 450 incident ischemic stroke cases and 450 matched controls from the Nurses’ Health Study.
  • Proteomic analysis using the Olink Explore HT platform to measure 5,440 proteins in plasma.
  • Weighted co-expression network analysis and functional gene set enrichment analysis were performed on proteins associated with stroke.
  • Fourteen proteins significantly associated with ischemic stroke risk (q value < 0.05).
  • The cluster most positively correlated with stroke risk was linked to aging and inflammation (r = 0.16).
  • Neutrophil degranulation was identified as the top pathway positively associated with ischemic stroke.

Study Design

Type

Case-Control (n=900)

Structured PICO

Are specific plasma proteomic profiles and pathways associated with incident ischemic stroke risk in women?

P
Population
900 women (450 incident ischemic stroke cases and 450 matched controls) from the Nurses' Health Study, median age 65.8
I
Intervention
Measurement of 5,440 plasma proteins using the Olink Explore HT platform
C
Comparator
Matched controls without incident ischemic stroke
O
Outcome
Incident ischemic strokehard clinical

Main Result

p-value: p=<0.05

Large-scale proteomic analysis identified 14 circulating proteins and distinct metabolic and inflammatory pathways associated with ischemic stroke risk in women.

Limitations

  • Findings require validation in external cohorts

Cite This Study

Sanchez et al. (2026) conducted a case-control in Ischemic stroke (n=900). Proteomic profiling vs. Matched controls was evaluated on Incident ischemic stroke (p=<0.05). Proteomic analysis of 5,440 proteins identified 14 proteins significantly associated with ischemic stroke risk in women (q<0.05), highlighting distinct metabolic and inflammatory pathways.

synapsesocial.com/papers/69fadaab03f892aec9b1e6d3https://doi.org/10.1161/cir.153.suppl_1.th982
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