Eco‐Friendly Analytical Approaches for the Quantification of Molnupiravir and Favipiravir Using Capillary Zone Electrophoresis and Derivative Ratio Spectrophotometry

Abstract Designing new validated analytical procedures with a focus on many objectives, such as simplicity, environmental friendliness, analytical effectiveness, and sustainability, has grown to be a top priority in quality control departments. Applying green and white analytical chemistry concepts is no longer a luxury. In this report, two new approaches, Capillary Zone Electrophoresis (CZE) with Photo Diode Array (PDA) detection and derivative ratio spectrophotometric methods, are established and validated for the simultaneous analysis of molnupiravir (MLP) and favipiravir (FAV) in bulk, single dosage forms, and their expected combined dosage forms. For the two drugs, linearity ranges of 5–50 μg/mL and 1–30 μg/mL were achieved using the CZE and spectrophotometric methods, respectively. Both techniques have been validated and used for the estimation of the two medications in lab‐made capsules in accordance with the International Council for Harmonization's (ICH) recommendations. Seven assessment models were used to evaluate the greenness and sustainability of the introduced approaches compared to three previously published HPLC, chemometric, and HPTLC techniques. ChlorTox and BAGI tools are applied for the first time for the evaluation of an analytical procedure. Higher green and sustainable qualities were declared by suggested approaches than by earlier ones.