P-1545. Impact of Perinatal Exposure and Colonization by Drug-resistant Enterobacterales on Infant Gut Microbiota

Abstract Background Antibiotic-resistant Enterobacterales, including beta-lactamase producing (BL-E) and ceftriaxone resistant (CefR-E) strains, are prevalent in the U.S. and globally. Our objective was to assess the impact of resistant Enterobacterales exposure and perinatal colonization on infant microbiota development in healthy parent-infant dyads.Table 1.Demographics and clinical dataFigure 1.Enterobacterales species prevalence and relative abundance in maternal and infant samples Methods Pregnant women were enrolled during labor at a high-volume hospital in Chicago, IL. Exclusion criteria were delivery 35 weeks gestation, fever, scheduled cesarean, and NICU admission. Maternal rectal swabs were collected prior to delivery, and infant stool was collected at 1-2 days (timepoint1), 7-10 days (t2), 2 months (t3), and 8 months of life (t4). Clinical data were obtained from the medical record and parental survey. Samples were plated on MacConkey + ceftriaxone and underwent shotgun next generation sequencing. Shotgun sequencing results were decontaminated and processed to obtain microbial taxonomic profiling and BL gene identification. CefR culture-based and metagenome-based results were combined to identify each sample’s beta-lactamase status (BL-E negative or positive). Whole genome sequencing was performed for ceftriaxone resistant (CefR) isolates.Figure 2.Prevalence and relative abundance of Escherichia coli in mother and infant gut microbiomeFigure 3.Beta diversity of infant gut microbiome over time grouped by BL-E status combining culture- and metagenome-based identificationKruskal-Wallis test p 0.01; post hoc pairwise comparisons using Wilcoxon rank sum test p 0.01 between all timepoints for infant samples Results Sixty-one dyads were enrolled 2/2022-1/2023 Table 1, with 57 parent and 185 infant stool samples (t1=46, t2=50, t3=46, t4=43). CefR-E was identified in 15 parent and 13 infant samples over the study period. Not all infants with CefR-E had CefR-E cultured at subsequent timepoint. Among the 35 Enterobacterales species identified, Escherichia coli showed the highest prevalence and relative abundance Fig 1. Infant E. coli relative abundance decreased over time. Infant E. coli prevalence showed an increasing trend over time, with a distinct difference between BL-E positive and negative samples Fig 2. Difference in infant microbial community structure and beta diversity between BL-E positive and negative samples was larger for early time points (t1 and t2) compared to later time points (t3 and t4) Fig 3. Conclusion Maternal and perinatal colonization with beta-lactamase resistant Enterobacterales leads to gut microbiome differences in early infancy. The impact of these early life alterations in E. coli and diversity warrants further investigation. Disclosures All Authors: No reported disclosures