How the methodology determines the outcome of the in vitro micronucleus assay (OECD TG 487): a comparison of the MicroFlow and the microscopic evaluation approach highlights the impact of cytotoxicity/cytostasis metrics in V79 cells for matrine
Comparative analysis shows the evaluation method influences micronuclei results in V79 cells, suggesting complications arise from cytotoxic metrics.
Key Points
This study aims to compare the efficacy of the MicroFlow and microscopic evaluation methods for the in vitro micronucleus assay, focusing on the impact of cytotoxicity metrics.
Conducted an in vitro micronucleus test using V79 cells with matrine and oxymatrine.
Used two evaluation methods: MicroFlow kit and microscopic analysis.
Tested multiple concentrations of matrine for 4 h and 24 h incubation periods.
Included mechanistic studies with ToxTracker ACE assay.
MicroFlow revealed significant increases in micronuclei formation starting from 1.0 mg/ml matrine, despite reduced cell survival.
Microscopic evaluation did not corroborate MicroFlow findings due to high cytotoxicity at similar concentrations.
Matrine was identified as not a direct clastogen based on mechanistic studies.