Hen egg Lysozyme fibrils act as amyloid nucleating agents for β-lactoglobulin and Insulin

Key Points

• This research aims to understand how hen egg lysozyme fibrils induce amyloid formation in other proteins.
• Investigated the interactions between hen egg lysozyme fibrils and proteins like β-lactoglobulin and insulin.
• Analyzed fibril formation using biochemical assays to observe cross-seeding effects.
• Employed microscopy techniques to visualize amyloid structure and aggregation.
• Lysozyme fibrils significantly enhance the amyloid formation of β-lactoglobulin.
• Insulin also shows increased aggregation in the presence of lysozyme fibrils.
• Findings suggest a mechanism for how amyloid diseases may propagate through cross-seeding.