Cellulomonas olei sp. nov., isolated from oil-contaminated soil

A bacterial strain, designated as P4 T , was isolated from an oil-contaminated soil sample. The cells were Gram-stain-positive, short rod-shaped and motile with a single lateral flagellum. Growth of the strain was observed at 20–40 °C (optimum at 30 °C), at pH 6.0–11.0 (optimum at pH 7.0) and with 0–5.0 % (w/v) NaCl (optimum at 1.0%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P4 T was closely related to the members of genus Cellulomonas , with the highest sequence similarity to Cellulomonas hominis DSM 9581 T (99.5%), followed by Cellulomonas endometrii Marseille-Q7820 T (99.1%), Cellulomonas pakistanensis NBRC 110800 T (99.0%) and Cellulomonas denverensis NBRC 110783 T (98.1%). The draft genome sequence of strain P4 T was 3.98 Mb in length, with a DNA G+C content of 75.5 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain P4 T and the closely related type strains were in the range of 78.6–84.4 % and 21.9–28.2 %, respectively. The predominant cellular fatty acids of strain P4 T included anteiso-C 15 :0 , C 16:0 , C 14:0 , iso-C 15:0 and anteiso-C 17:0 . The main polar lipid was composed of diphosphatidylglycerol, glycophospholipid, glycolipid and three phosphatidylinositol mannosides and the major menaquinone was MK-9(H4). Based on the polyphasic characterization, strain P4 T (=KCTC 49393 T =CCTCC AB 2019402 T ) represents a novel species of the genus Cellulomonas , for which the name Cellulomonas olei sp. nov. is proposed.