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January 22, 2026F1000Research0 citationsOpen Access

Comparative Analysis of Manual and Automated (HbA1c) Methods in β-Thalassemia Carriers with Diabetes

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OAOmar NK AlheetiONOthman NajeebMFMustafa M. Fahad

Key Points

  • This analysis aims to compare the effectiveness of manual and automated methods in measuring HbA1c levels in diabetic β-thalassemia carriers.
  • Compared manual HbA1c testing with automated HPLC technique.
  • Included 60 diabetic cases from Fallujah Teaching Hospital.
  • Analyzed HbA1c using three methods: Variant II HPLC, ion-exchange resin, and Turbidimetric immunoassay.
  • Utilized ANOVA, t-test, and Pearson correlation for data analysis.
  • Automated HPLC showed a higher mean HbA1c level (9.2%) than Turbidimetric immunoassay (7.58%) and ion-exchange resin (7.96%).
  • Significant differences in measurements reaching 1.5% and 1.15% for automated and manual methods, respectively.
  • Strong positive correlation found between HbA1c and HbA values across all methods (r > 0.82, p < 0.001).

Abstract

Background Hemoglobin A1c is widely recognized as an indicator of long-term glucose control in patients with diabetes. Its measurement, however, can be influenced by several hemoglobin disorders, including the β-thalassemia trait, which is relatively common in the Middle East. Methods This work compared manual HbA1c testing procedures with an automated high-performance liquid chromatography (HPLC) technique in diabetic patients carrying the β-thalassemia trait. Sixty diagnosed diabetic cases with confirmed β-thalassemia were included from Fallujah Teaching Hospital. HbA1c was analyzed by three procedures: Variant II HPLC, ion-exchange resin, and Turbidimetric immunoassay. Data were processed using ANOVA, t-test, and Pearson correlation in SPSS, considering p 0.82, p

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Cite This Study

Alheeti et al. (2026) studied this question.

synapsesocial.com/papers/6971bd90642b1836717e22a9https://doi.org/10.12688/f1000research.174502.1
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