Effects of Different Electric-Field Intensities on Nitrogen Transformation and Bacterial Community Structure During Biochar Aerobic Composting

In this study, the effects of electric-field intensity on N transformation during aerobic composting of biochar/pig manure were investigated. Four experimental groups were established under different applied voltages: 0 V (Group CK) ; 2 V (Group L) ; 4 V (Group M) ; and 5 V (Group H). The physicochemical properties of compost, as well as the nitrogen content and its existing forms in the compost, were systematically analyzed. The underlying mechanisms were further explored from the microscopic perspective by analyzing the pore structure of biochar and the microbial diversity in compost. The results showed that the total nitrogen content in compost increased by 5. 66–20. 87% with the application of the electric field. Cumulative NH3 emissions decreased by 37. 43%, 31. 35%, and 40. 95% in groups L, M, and H, respectively, while the NO2 content decreased by 40. 73%, 87. 93%, and 94. 44%, respectively, reducing the N losses during composting. The electric field significantly promoted the migration of nutrients from the compost to the surface of cotton stalk biochar. It also enhanced the microporous structure and adsorption capacity of cotton stalk biochar, thereby facilitating interfacial deposition and N immobilization. The amplification and sequencing of 16S rRNA gene further revealed that Ruminofilibacter, norankfMWH-CFBk5, and HN-HF0106 were the key bacterial genera affecting the gas emissions during aerobic composting. Among them, Ruminofilibacter and HN-HF0106 promoted the emission of N2O, while norankfMWH-CFBk5 and Planktosalinus reduced NH3 emission. This finding indicates that the electric field regulated N transformation and promoted N retention in compost by inhibiting the reproduction of denitrifying bacteria and increasing the abundance of nitrifying and nitrogen-fixing bacteria. This study confirms that electric field and biochar synergistically affect the nitrogen immobilization and waste resource utilization by optimizing the metabolic pathways of microorganisms and the structural characteristics of biochar.