Efficient Quantification of Methazolamide in Rat Plasma Using UHPLC-Q-Orbitrap MS for Pharmacokinetic Analysis

Key Points

• The research aims to develop a reliable method for measuring methazolamide in rat plasma to understand its pharmacokinetics.
• Utilized ultra-high performance liquid chromatography coupled with orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS).
• Analyzed plasma samples from rats after methazolamide administration.
• Established calibration curves for accurate quantification.
• Conducted pharmacokinetic analysis based on the collected data.
• Developed a sensitive and efficient method for quantifying methazolamide.
• Identified key pharmacokinetic parameters of methazolamide in rat plasma.
• Demonstrated the method's applicability for further pharmacological studies.