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January 23, 2026npj Biofilms and MicrobiomesOpen Access

Probiotic bacteria Bifidobacterium bifidum upregulation of intestinal epithelial tight junction barrier is mediated by TLR-2/TLR-6 receptor complex activation of occludin gene

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Authors

RARaz AbdulqadirRARana Al–SadiYGYash Gupta

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Overview

Investigates the mechanism of Bifidobacterium bifidum enhancing the intestinal barrier in inflammatory bowel disease, suggesting beneficial microbe-host interactions.

Key Points

  • To explore how Bifidobacterium bifidum upregulates the intestinal epithelial tight junction barrier.
  • Used in vitro models (Caco-2 monolayers) and in vivo models (live mice) to study the effects.
  • Measured occludin gene activity and protein expression levels.
  • Evaluated the role of TLR-2/TLR-6 receptor complex activation in the process.
  • Bifidobacterium bifidum upregulated the intestinal tight junction barrier, specifically increasing occludin levels.
  • Knockdown of occludin prevented the enhancement of the tight junction barrier.
  • The mechanism involved TLR-2/TLR-6 activation and IRAK-1 phosphorylation leading to TOLLIP recruitment.

Cite This Study

Abdulqadir et al. (2026) studied this question.

synapsesocial.com/papers/69730f78c8125b09b0d1f450https://doi.org/10.1038/s41522-025-00903-7
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