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January 23, 2026Journal of Crohn s and Colitis

P0082Mesenteric inflammation-associated fibroblasts induce intestinal epithelial barrier dysfunction and Crohn’s disease progression

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Authors

JPJ ParkYJY H JooESE M Song

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Overview

Demonstrates that mesenteric fibroblasts worsen epithelial barrier function in Crohn’s disease, suggesting new treatment possibilities.

Key Points

  • The study investigates the role of mesenteric inflammation-associated fibroblasts in Crohn's disease progression and epithelial barrier dysfunction.
  • Isolated mesenteric IAFs and normal fibroblasts from patients with Crohn’s disease and control samples.
  • Analyzed cell viability, invasion, and collagen contraction through assays.
  • Used transwell and 3D Matrigel co-cultures to assess fibroblast effects on colonic epithelial cancer cell lines.
  • Conducted RNA-seq followed by gene set enrichment and gene ontology analyses.
  • IAFs showed increased cell growth, wound healing, invasion, and collagen contraction compared to normal fibroblasts.
  • IAFs induced abnormal lumen formation in Caco-2 cell spheres in 3D cultures.
  • Promoted epithelial barrier disruption was noted in transwell co-culture assays.
  • Transcriptomic analyses revealed upregulated chemokine and cytokine pathways specific to IAFs.

Cite This Study

Park et al. (2026) studied this question.

synapsesocial.com/papers/69731005c8125b09b0d1fc5chttps://doi.org/10.1093/ecco-jcc/jjaf231.263
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