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January 23, 2026Royal Society Open Science0 citationsOpen Access

Genomic insights reveal zoonotic potential of Morganella morganii strains from diarrhoeal patients in Dhaka, Bangladesh

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FKFahad KhanSSSenzuti SharminARAura Rahman

Key Points

  • This research investigates the genomic features and antimicrobial resistance of Morganella morganii strains from diarrhoeal patients and environmental sources in Dhaka, Bangladesh.
  • Conducted whole-genome sequencing on seven isolates from diarrhoeal stool samples and three environmental samples.
  • Analyzed antimicrobial resistance profiles and identified resistance genes.
  • Utilized interolog-based predictions to assess host-pathogen protein–protein interactions.
  • Performed phylogenomic analysis to determine the relationship between isolates and potential zoonotic transmission.
  • Identified diverse resistance genes, including β-lactamases and fluoroquinolone resistance genes.
  • Found 3920 potential host-pathogen protein–protein interactions involved in immunomodulatory capabilities.
  • Revealed phylogenomic clustering of clinical isolates with strains from animals and environmental sources, suggesting zoonotic transmission.

Abstract

Abstract Morganella morganii, a Gram-negative opportunistic pathogen, is increasingly recognized as a cause of nosocomial and community-acquired infections; however, comprehensive genomic studies from Bangladesh remain limited. In this study, seven M. morganii isolates from diarrhoeal stool samples and three from environmental sources in Dhaka were subjected to whole-genome sequencing to investigate their genomic diversity, antimicrobial resistance (AMR) profiles, virulence factors and host–pathogen protein–protein interactions (HP-PPIs). Genome sizes ranged from 3.75 to 4.09 Mbp, with an average GC content of approximately 51%. AMR analysis revealed diverse array of resistance genes, including β-lactamases (blaCTX-M-15, blaOXA-1), aminoglycoside-modifying enzymes (aadA5, aph(6)-Id, aac(6’)-Ib-cr), fluoroquinolone resistance genes (qnrB4,), sulfonamide resistance genes (sul1, sul2) and tetracycline resistance determinants (tet(A), tet(D)). Interolog-based predictions identified 3920 potential HP-PPIs, including bacterial transketolase interacting with human NF-κB (NFKB1), suggesting immunomodulatory capabilities. Importantly, phylogenomic analysis revealed clustering of clinical isolates with strains from animal and environmental sources, indicating potential zoonotic transmission of M. morganii. This study reveals key genomic and phenotypic features of M. morganii in Bangladesh and underscores the importance of surveillance and targeted control strategies for this emerging pathogen.

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Cite This Study

Khan et al. (2026) studied this question.

synapsesocial.com/papers/6973106cc8125b09b0d201dchttps://doi.org/10.1098/rsos.251259
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