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January 24, 2026Advanced ScienceOpen Access

Systematic Metabolic Engineering and Model‐Guided Optimization for High‐Level Production of L‐Theanine From Xylose in Escherichia Coli

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Authors

HHHaolin HanBXBoyuan XueGSGuangqi Shan

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Overview

Systematic optimization produces high yields of L-theanine from xylose, suggesting a novel biomanufacturing pathway.

Key Points

  • The aim is to develop an efficient microbial platform for producing L-theanine from xylose using E. coli.
  • Reconstructed the Weimberg pathway in E. coli for L-theanine biosynthesis from xylose.
  • Blocked competitive metabolic pathways and enhanced precursor supply.
  • Used flux balance analysis to assess metabolic networks.
  • Implemented a two-stage microaerobic-aerobic cultivation strategy.
  • Conducted fed-batch fermentation of the engineered strain.
  • Achieved a titer of 14.31 g/L of L-theanine and a yield of 0.48 g/g xylose.
  • The overproducing strain reached a titer of 95.42 g/L and a productivity of 1.33 g/L/h.
  • Represented a 2811.4-fold increase in titer compared to the original strain.

Cite This Study

Han et al. (2026) studied this question.

synapsesocial.com/papers/6974616cbb9d90c67120b43chttps://doi.org/10.1002/advs.202521440
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