PulseExploreJournal ClubDebatesTrendingResearchersJournals
Instagram
HomeExploreJournal ClubTrending
Synapse
⌘+K
Synapse
January 24, 20260 citations

Designing Bifunctional Antibodies for siRNA Delivery Using Staufen dsRNA-Binding Domain

Staufen dsRNA-binding domain as modules to design bifunctional antibodies for siRNA delivery.

View Full Paper
Ask AI
Bookmark
Share

Authors

YLYing LiuYZYan ZhengRXRuolin Xu

Discussion

Loading...

Member takes

Overview

Research demonstrates enhanced siRNA delivery with bifunctional antibodies in various cell types, indicating potential for broader therapeutic use.

Key Points

  • This research aims to develop bifunctional antibodies that effectively deliver siRNA for gene silencing in extrahepatic tissues.
  • Designed bifunctional antibodies with Staufen1 dsRBD34 for siRNA recruitment.
  • Tested the siRNA-antibody pairing across multiple cell types for target gene silencing.
  • Conducted affinity maturation via arginine scanning to enhance dsRNA-binding affinity.
  • Assessed silencing potency changes with small-molecule enhancers.
  • Evaluated pharmacokinetics of paired systems in circulation.
  • Achieved 1:1 stoichiometry in siRNA-antibody pairing.
  • Increased silencing potency of siRNA by seven times with terminal enhancements (IC50 reduced from 200 to 28 nM).
  • Affinity maturation resulted in a 32-fold increase in binding affinity to siRNA.
  • Sulfated GAGs significantly affected pharmacokinetics, complicating in vivo assessments.
  • Bifunctional antibodies demonstrated potential but required ultrahigh-affinity domains for practical application.

Cite This Study

Liu et al. (2026) studied this question.

synapsesocial.com/papers/6974616cbb9d90c67120b4adhttps://doi.org/10.1093/nar/gkaf1539
View Full Paper
Ask AI
Bookmark
Share