A surrogate‐selection‐enhanced TnpBmax platform enables efficient genome editing in rice and tomato

Key Points

• The aim is to enhance genome editing efficiency in rice and tomato using a new optimized platform.
• Utilized TnpB, a RNA-guided nuclease, optimized for plant genome editing
• Developed TnpBe5 by fusing enhanced RNA with T5 exonuclease
• Coupled TnpBmax with a HYYG surrogate for enriched cell selection
• Achieved a 2.5-fold increase in editing efficiency in rice
• Reached up to 81.5% editing efficiency in selected cells
• Ensured high homozygosity in genome-edited rice and tomato plants