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February 2, 20260 citationsOpen Access

Morphological and molecular evidence support a new species of the genus Leptobrachella (Anura, Megophryidae) from central China

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LJLiming JiangJLJing LiuYHYun Huang

Key Points

  • The research aims to identify and describe a new species of the genus Leptobrachella based on morphological and molecular data.
  • Morphological comparisons of specimens from Xinshao County, Hunan Province, China.
  • Molecular phylogenetic analyses utilizing mitochondrial 16S rRNA and COI gene sequences.
  • Calculation of uncorrected p-distances to assess genetic divergence from closest congeners.
  • The new species is distinct from its congeners with p-distances of 2.1% and 8.1% for 16S rRNA and COI genes.
  • Morphological features include a specific SVL range of 27.8–31.4 mm in males and rudimentary webbing on toes.
  • Characterized by distinct tubercles around the tympanum and a shagreened dorsal surface with small, raised tubercles.

Abstract

Based on morphological comparisons and molecular phylogenetic analyses, a new species of the genus Leptobrachella from Xinshao County, Hunan Province, China, is described. Phylogenetic analyses of mitochondrial 16S rRNA and COI gene sequences support the new species as an independent lineage. The uncorrected p-distances of the 16S rRNA and COI genes between the new species and its closest congeners are 2.1% and 8.1%, respectively. The new species is distinguished from its congeners by a combination of the following characters: (1) SVL 27.8–31.4 mm in males; (2) toes rudimentarily webbed, with narrow lateral fringes; (3) tympanum distinct, surrounded by distinct tubercles; (4) dorsal surface shagreened, with small, raised tubercles and longitudinal ridges; (5) ventral surface creamy white, with dark brown spots on the chest and margins; (6) distinct black spots present on the flanks; (7) heels not meeting when hindlimbs are flexed at right angles to the body axis; and (8) tibia–tarsal articulation reaching between the eye and the tympanum.

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Cite This Study

Jiang et al. (2026) studied this question.

synapsesocial.com/papers/6980fc37c1c9540dea80df78https://doi.org/10.3897/zse.102.175462
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