Pleurotus djamor strain PL01‐White is a locally occurring white oyster mushroom strain in Sri Lanka. It is widely appreciated as a culinary delicacy and is also recognized for its notable medicinal properties. In the present study, the antimicrobial, antioxidant, and antihyperglycemic activities of P. djamor strain PL01‐White were studied, and the whole genome of the mushroom was sequenced to relate phenotypic data with the genotype. The mushroom exhibited antimicrobial activity against Escherichia coli , Salmonella enterica serovar Typhi, and Salmonella enterica serovar Paratyphi in a well‐diffusion assay. The half maximal effective concentration (EC 50 ) value in the DPPH scavenging assay for mushroom ethanol extract was 22.81 µg/mL while 21.96 µg/mL for a known antioxidant, ascorbic acid, showing a comparatively high antioxidant potential in the mushroom. The mushroom hot water extract showed a half maximal inhibitory concentration (IC 50 ) value of 487 µg/mL in α ‐amylase inhibition assay and 537 µg/mL in α ‐glucosidase inhibition assay, demonstrating significant antihyperglycemic potential. The whole genome of the mushroom was sequenced using the Illumina platform with 59.3 Mb of assembly size. A total of 21,707 genes have been annotated using homology‐based, transcriptome‐based, proteomic‐based, and ab initio tools. The potential secondary metabolite‐producing gene clusters of the genome were identified using the tool antiSMASH, and several gene clusters were identified, leading to the production of secondary metabolites with antimicrobial, antioxidant, and antihyperglycemic activities. Given its identified and confirmed medicinal properties, this mushroom has strong potential for development as a nutraceutical and for the discovery of novel pharmaceutical compounds.
Dharmasena et al. (2026) studied this question.