This study aimed to investigate the effects of dietary supplementation with microalgae, herbal powder, and conjugated linoleic acid (CLA) on production performance, blood parameters, and ovarian activity in Loh-man laying hens. Sixty 43-week-old hens (1.6±0.2 kg) were randomly assigned to four dietary groups for 42 days: a control group (no additives) and three experimental groups receiving 0.1%, 0.2%, or 0.3% of a supplement blend containing Spirulina, Chlorella, CLA, and herbal powders (dill and peppermint). Parame-ters including production performance, egg weight, serum lipids, ovarian follicle activity, and liver fat were evaluated. Results indicated that the highest supplementation level (0.3%) significantly improved produc-tion performance and egg weight compared to the control and other groups (P<0.05). On day 42, serum lipid analysis showed a significant reduction in cholesterol levels in the 0.3% group (P<0.05). Ovarian fol-licle activity increased with higher supplementation levels, showing a statistically significant enhancement over the control (P<0.05). Liver evaluation revealed a notable decrease in total liver fat in the supplemented groups, particularly at the 0.3% level (P<0.05). In conclusion, dietary inclusion of 0.3% microalgae, CLA, and herbal supplements enhanced productive performance, improved egg quality, optimized serum choles-terol levels, supported ovarian activity, and contributed to better liver health in Lohman laying hens.
Chashnidel et al. (2025) studied this question.