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February 5, 2026Small Methods0 citations

Highly Loaded Erlotinib Tubular Micromotor for Targeted Drug Delivery, pH Responsive Release and Ultrasound Tracking: A Three‐In‐One Micromachine

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XLXiaochun LiKLKaiying LiangKYKaisong Yuan

Key Points

  • The research aims to create a multifunctional micromotor for targeted drug delivery of erlotinib and evaluate its performance.
  • Fabrication of a calcium carbonate tubular micromotor using an internal-filling strategy.
  • Loading the micromotor with erlotinib microparticles.
  • Testing the micromotor's propulsion capabilities and drug release under acidic conditions.
  • Evaluating the micromotor's efficacy on non-small cell lung cancer cells (A549).
  • Achieved a drug payload of 2.53 × 10−12 mol per micromotor.
  • Demonstrated effective pH-responsive drug release under acidic conditions.
  • Micromotor exhibited real-time tracking capabilities using generated bubbles as ultrasonic contrast agents.
  • Showed potential anti-cancer efficacy by killing A549 cells.

Abstract

ABSTRACT Herein, we report a novel erlotinib (EH) ‐loaded calcium carbonate (CaCO 3 ) tubular micromotor fabricated via an internal‐filling strategy, achieving a high drug payload of 2.53 × 10 − 1 2 mol per micromotor, which integrates three core functionalities in one system: targeted delivery of EH, pH‐responsive release, and ultrasound‐based tracking. The microtube structures (10 µm in diameter) are prepared by electrochemical deposition, followed by filling the EH@CaCO 3 microparticle into the interior of the tubular motor. Distinct from the surface coating approach for drug immobilization, this internal‐filling strategy enables substantially greater payloads. The EH@CaCO 3 tubular micromotor shows favorable bubble and magnetic propulsion capabilities. Serving as a proof‐of‐concept for targeted anti‐cancer drug delivery, these micromotors can transport drugs within microchips channel to the targeted position. Under acidic conditions, CaCO 3 undergoes decomposition to release the encapsulated drug. Concurrently, the Zn‐based inner structure of the tubular micromotor reacts with hydrogen ions (H + ), leading to micromotor degradation and thereby facilitating rapid drug release. The as‐released drug shows cell killing ability toward non‐small cell lung cancer cells A549. Meanwhile, as the micromotors move in an acidic environment, the in situ generated bubbles can act as “ultrasonic contrast agents”, thereby enabling real‐time tracking of the micromotors. For potential in vivo applications, this facilitates the tracking of such motors in scenarios where optical microscopy is ineffective. The blood compatibility, coagulation function, and preliminary in vivo immune response evaluation all indicate that the system had good biosafety. This study provides a new idea for the development of a next‐generation micro drug delivery platform with high drug loading, intelligent delivery, and real‐time visualization.

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Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/69843398f1d9ada3c1fb0cfdhttps://doi.org/10.1002/smtd.202501926
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