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February 5, 2026Analytical Methods0 citations

Single-step Duplex CRISPR Coupled with Lateral Flow Assay for Point-of-care Detection of Human Immunodeficiency Virus and Treponema Pallidum

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ZXZiwen XuMWMuyun WeiMJMingming Jiang

Key Points

  • To develop a rapid and sensitive method for detecting HIV and Treponema pallidum using a CRISPR-lateral flow assay.
  • Developed a single-step duplex CRISPR technique
  • Utilized lateral flow assay for point-of-care application
  • Tested sensitivity and specificity against known samples
  • Achieved high sensitivity and specificity in detecting HIV and Treponema pallidum
  • Demonstrated potential for rapid testing in clinical settings

Abstract

The continued acquired immunodeficiency syndrome (AIDs) caused by human immunodeficiency virus (HIV) and syphilis caused by Treponema pallidum (TP) posed great challenge for global health, highlighting the rapid and sensitive...

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Cite This Study

Xu et al. (2026) studied this question.

synapsesocial.com/papers/698433a5f1d9ada3c1fb0f2dhttps://doi.org/10.1039/d5ay02168e
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