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Synapse
February 5, 20260 citations

Calibration of an in-line digital holographic microscope

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HRHannes RobbenRCReinhard CasparyKBKonrad Bethmann

Key Points

  • The research aims to develop a calibration method for digital holographic microscopes integrated into two-photon polymerisation systems.
  • Developed a calibration method correlating in-line measurements to offline high-resolution results.
  • Integrated digital holographic microscope measurements with two-photon polymerisation.
  • Validated calibration using structures created with a specific polymer.
  • A single calibration step is sufficient for reliable monitoring of various structures.
  • The method facilitates real-time adaptation of the production process to geometry changes.

Abstract

We present a calibration method that correlates in-line measurement results from a digital holographic microscope (DHM) integrated into a two-photon polymerisation system (TPP) with offline results from a high-resolution laser scanning microscope (LSM). Our findings indicate that a single calibration step for a specific polymer is sufficient to reliably monitor the fabrication of various structures. In future, this approach will make it possible to adapt the production process to the manufactured structure geometry in real time.

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Cite This Study

Robben et al. (2025) studied this question.

synapsesocial.com/papers/69843451f1d9ada3c1fb2525https://doi.org/10.1051/epjconf/202533501003/pdf
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Enhancement and evaluation of phase imaging capabilities in lensless digital holographic microscopy2024
  2. 2Joint estimation of calibration bead’s parameters and monochromatic aberrations in in-line digital holographic microscopy2024
  3. 3Thermal-light self-reference digital holographic microscopy with submicron resolution2026
  4. 4Lensless single-shot dual-wavelength digital holography for industrial metrology2024 · 9 citations
  5. 5Dimensionally Traceable 3D Microstructures for Multimodal Microscope Calibration2026