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February 5, 2026Journal of Eukaryotic Microbiology0 citationsOpen Access

Genetic Features of the Scuticociliate Pathogen Philaster sp. Isolate FWC2 That Causes Sea Urchin Mass Mortality

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SLShen Jean LimMBMya Breitbart

Key Points

  • The research aims to analyze the genetic features of the scuticociliate pathogen Philaster sp. isolate FWC2.
  • Cultured the scuticociliate related to Philaster apodigitiformis.
  • Sequenced the metagenomic content to assess the genome size.
  • Assembled and annotated the nuclear ribosomal operon and mitochondrial genome.
  • Predicted genome size of Philaster sp. isolate FWC2 is 122–136 Mbp.
  • Annotated a 4,088 bp nuclear ribosomal operon and a 41,396 bp mitochondrial genome.
  • Identified a protein sequence homologous to β‐PKA, potentially involved in host infection.

Abstract

ABSTRACT A scuticociliate most closely related to Philaster apodigitiformis caused mass mortalities of diadematoid sea urchins and was cultured as Philaster sp. isolate FWC2. We sequenced the metagenomic content of this isolate, which was predicted to represent ≤ 56% of the complete genome. Based on k ‐mer counts, the haploid genome size was predicted to be 122–136 Mbp. We assembled and annotated a 4,088 bp nuclear ribosomal operon, a 41,396 bp mitochondrial genome with 19.22% G + C content, 24 protein‐coding genes, 6 tRNA genes, and 2 rRNA genes, and a protein sequence homologous to β‐PKA in Philaster apodigitiformis potentially involved in host infection.

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Cite This Study

Lim et al. (2026) studied this question.

synapsesocial.com/papers/6984345ff1d9ada3c1fb27achttps://doi.org/10.1111/jeu.70065
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