Covalent targeting of PSMD14 by Eupalinolide B induces oncoprotein degradation and apoptosis in acute promyelocytic leukemia cells

Key Points

• The aim is to explore how Eupalinolide B impacts PSMD14 and affects oncoprotein levels in acute promyelocytic leukemia cells.
• Utilized Eupalinolide B to target PSMD14 covalently
• Assessed deubiquitinase activity
• Measured levels of oncoproteins AKT1 and CDK4
• Evaluated cell cycle progression and apoptosis rates
• PSMD14 was successfully inactivated by Eupalinolide B
• AKT1 and CDK4 levels decreased significantly
• Cell cycle arrest occurred following treatment
• Apoptosis was induced in leukemia cells as a result of cascaded effects

Abstract