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February 8, 2026SHILAP Revista de lepidopterología0 citationsOpen Access

The Impact of Water Storage on Microleakage of Two Generations of Dentin Bonding Agents: An In-Vitro Study

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ZMZahra Agha Mohammadi

Key Points

  • This study aims to evaluate the microleakage levels of different self-etch adhesives in composite restorations depending on water storage time.
  • Prepared standard Class V cavities on 84 human premolars
  • Divided samples into six groups of bonding agents
  • Filled cavities with Clearfil AP-X after bonding procedures
  • Stored samples in deionized water for 24 hours and 6 months
  • Analyzed microleakage using Kruskal-Wallis and Mann-Whitney U tests
  • Microleakage at the occlusal surface was greater than at the cervical surface for the SE group after 24 hours
  • No significant differences in microleakage between occlusal and cervical borders were found for PB and TS groups after 24 hours
  • SE and TS groups showed no leakage at both surfaces after 6 months of water storage

Abstract

Objectives The latest generation of bonding agents is increasingly embraced and well-suited to fulfilling the requirements for durable restorations. This study aimed to assess microleakage levels of three self-etch adhesives (Clearfil SE (SE), Protect (PB), and Tri-S (TS) Bonds) in composite restorations at both occlusal and cervical borders, while considering the impact of water storage over different time intervals. Methods Standard Class V cavities were prepared on buccal surfaces of 84 human premolars at the level of cementoenamel junction. The samples were randomly assigned to six groups (N=14). The corresponding bonding procedure in each group was completed, and the cavities were filled using Clearfil AP-X (Kuraray). Following thermocycling, the teeth were stored in deionized water within an incubator for 24-hour and 6-month intervals. Statistical analysis of microleakage data was conducted utilizing Kruskal-Wallis and Mann-Whitney U tests at p<0.05. Results Following a 24-hour interval, the level of microleakage at the occlusal surface was greater than the gingival surface for SE. No statistically significant differences were observed in microleakage at the occlusal and cervical borders after 24h of water storage for the PB and TS groups (P=0.51, 0.16). None of the SE and TS group samples showed leakage at the occlusal or cervical margins following a 6-month interval. Conclusion Self-etching bonding agents indicated increased occlusal microleakage compared to the cervical margin microleakage.

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Zahra Agha Mohammadi (2024) studied this question.

synapsesocial.com/papers/698827670fc35cd7a88462d1https://doi.org/10.22037/jds.v42i4.45371
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