Whole-genome analysis reveals Salmonella serovars linked to osteomyelitis in children with sickle cell disease, suggesting a need for better surveillance.
Key Points
To analyze Salmonella isolates from osteomyelitis cases in children with sickle cell disease in Senegal.
Confirmed Salmonella isolates using MALDI-TOF biotyping.
Performed antimicrobial susceptibility testing via disc diffusion method.
Executed whole-genome sequencing on an Illumina platform.
Conducted bioinformatic analyses with open-access tools.
Isolates identified belonged to seven serovars, predominantly Enteritidis and Typhimurium.
11 isolates were fully susceptible to tested antibiotics, while one was extensively drug-resistant.
Most isolates carried significant virulence factors, including Salmonella Pathogenicity Islands.