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February 8, 2026Успехи современной биологии / Advances in Current Biology0 citations

Establishing a Collection of Bacteria that Mimic Human Infectious Agents in Order to use Them for the Development of Rapid Antibiotic Resistance Testing Methods

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EEE.Yu. Epova

Key Points

  • The study aims to create a collection of bacterial isolates that mimic human infectious agents to improve rapid antibiotic resistance testing.
  • Collected 25 bacterial isolates from various plants and organisms.
  • Identified taxonomic affiliations using complete 16S ribosomal DNA sequencing.
  • Assessed antibiotic resistance against five antibiotics: kanamycin, ampicillin, spectinomycin, erythromycin, and chloramphenicol.
  • Identified strains with varying resistance profiles to tested antibiotics.
  • Some isolates were resistant to multiple antibiotics, while others were not resistant at all.
  • Facilitated comparison of rapid testing methods across different bacterial groups.

Abstract

A collection of 25 bacterial isolates has been picked up from the leaves of alfalfa , culvars Kamis and Bagryany, winter rapeseed , tulip sp., green parts of , celandine and Sarepta mustard , as well as the red poultry mite and soil nematode . There are 7 representatives of the genus , and among the isolates; 5 isolates of the genus , 2 isolates of the genus , 8 isolates of the genus , and representatives of the type of Actinomycetota: and . Identification of the taxonomic affiliation of the isolates was carried out by sequencing of complete 16S ribosomal DNA genes, which are deposited in the GenBank database. The degree of resistance of isolated isolates to five common antibiotics was studied: kanamycin (Km), ampicillin (Ap), spectinomycin (Sp), erythromycin (Em) and chloramphenicol (Cm). Strains resistant to several antibiotics, to individual antibiotics, and are not resistant to any of the tested antibiotics have been identified within each taxonomic group. This makes possible comparison of the effectiveness of rapid antibiotic resistance testing methods on several groups of bacteria.

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Cite This Study

E.Yu. Epova (2025) studied this question.

synapsesocial.com/papers/698828100fc35cd7a8847479https://doi.org/10.7868/s3034634725040017
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