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February 8, 2026CellsOpen Access

Temperature-Dependent and Semi-Quantitative Enzyme Profiles of Malacosoma disstria (Lepidoptera: Lasiocampidae) Hemocytic Cell Lines

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Authors

PGPaschalis GiannoulisHKHelen Kalorizou

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Overview

Insect cell lines reveal enzyme dynamics in response to temperature and microbial interactions, indicating immune signaling patterns.

Key Points

  • This research aims to characterize the enzyme profiles and growth conditions of Malacosoma disstria hemocytic cell lines.
  • Characterized growth temperatures of insect hemocytic cell lines.
  • Assessed enzyme secretion profiles from hemocyte cultures at 50% confluency.
  • Monitored morphological changes induced by proteases in modified Grace's medium.
  • Examined interactions with polystyrene and microbial surfaces.
  • UA-Md221 and Md108 optimal growth at 28 °C; UA-Md203 and Md66 at 21 °C.
  • Md108 exhibits broader temperature tolerance compared to other cultures.
  • Enzyme release profile includes increased activities of alkaline and acid phosphatases, esterases, and lipases.
  • Exposure to dead Bacillus subtilis altered enzyme secretion in Md66 and Md108.

Cite This Study

Giannoulis et al. (2026) studied this question.

synapsesocial.com/papers/698828990fc35cd7a88483e7https://doi.org/10.3390/cells15030302
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