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February 8, 2026DNA Research0 citationsOpen Access

Chromosome-level genome assembly of the Gerbera ( Gerbera hybrida ) using HiFi long-read and Hi-C technologies

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YAYo AoyagiRSRyo ShimadaHHHideki Hirakawa

Key Points

  • The aim is to create a detailed genome assembly and annotation for Gerbera hybrida to enhance molecular research.
  • Utilized PacBio high fidelity (HiFi) sequencing and Omni-C chromatin capture sequencing.
  • Developed both nuclear and organellar genome assemblies for G. hybrida.
  • Conducted syntenic analysis comparing G. hybrida to other Asterales genomes.
  • Achieved a nuclear genome assembly of 2.32 gigabases with 99.3% assembled into 25 scaffolds.
  • Identified 36,160 protein-coding genes and 11,572 non-coding transcripts in the nuclear genome.
  • Mitochondrial genome spans 363,511 bp with 36 protein-coding genes, while the chloroplast genome is 151,898 bp with 85 protein-coding genes.
  • Demonstrated that Gerbera has undergone whole-genome triplication through syntenic analysis.

Abstract

Abstract Gerbera hybrida is one of the most popular ornamental plants and also serves as a valuable model plant within the Asteraceae family. Here, we report both the nuclear and organellar genome assemblies and annotations of G. hybrida, which was developed through hybridization of two wild species. Sequencing was performed using a combination of PacBio high fidelity (HiFi) reads and chromatin capture reads (Omni-C). The total span of the nuclear genome assembly is 2.32 gigabases, and 99.3% of the sequence assembled into 25 scaffolds, consistent with the known chromosome number. Genome annotation of the nuclear genome identified 36,160 protein-coding genes and 11,572 non-coding transcripts. The mitochondrial genome had 363,511 bp and contains 36 protein-coding genes, 3 rRNAs, and 21 tRNAs, while the chloroplast genome is 151,898 bp in length and includes 85 protein-coding genes, 8 rRNAs, and 37 tRNAs. A syntenic analysis of the G. hybrida genome and published Asterales genomes demonstrated that Gerbera has undergone a whole-genome triplication. This reference genome provides a foundational resource for future molecular breeding and genetic research in Gerbera and the broader Asteraceae family.

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Cite This Study

Aoyagi et al. (2026) studied this question.

synapsesocial.com/papers/6988291e0fc35cd7a8849221https://doi.org/10.1093/dnares/dsag002
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