ABSTRACT Glioblastoma is the most common primary malignant brain tumor with a 5‐year survival rate 1 mm) to limit spatial distributions of hypoxia and potentially screen therapeutics in a controlled and physiologically relevant environment. Here, we establish a method to encapsulate GBM cells in gelatin and polyethylene glycol (PEG) microgels. We show that microgel composition can affect cell morphology and further, that collections of GBM‐laden hydrogels can be used to quantify the effect of single versus metronomic doses of TMZ. GBM metabolic activity is maintained in microgel culture and GBM cells display drug response kinetics similar to previously established literature using macro‐scale hydrogel constructs. Finally, we show microgels can be integrated with a liquid handler to enable high‐throughput screening using cell‐laden microgels.
Payan et al. (Sun,) studied this question.