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February 12, 2026Российская сельскохозяйственная наука / Russian Agricultural Sciences0 citations

Influence of Nutrient Medium and AIr Temperature on Development of Sclerotia of (Lib.) De Bary in Laboratory

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OSO.A. Serdyuk

Key Points

  • The aim is to understand how different nutrient media and temperatures affect sclerotia growth of a specific fungus.
  • Conducted laboratory experiments in 2024 with four nutrient medium and temperature combinations.
  • Used potato-glucose agar and Czapek's agar at temperatures of 25 °C and 27 °C.
  • Examined germination rates and mycelium growth of the fungus.
  • Collected sclerotia from winter rapeseed and white mustard plants in 2023 and 2024, respectively.
  • Sclerotia germination on potato-glucose agar was faster than mycelium growth on Czapek's agar, by 1.5 times at 25 °C and 3.6 times at 27 °C.
  • Using Czapek's agar reduced the number of new sclerotia formed by 28-35%.

Abstract

The research was conducted to study the peculiarities of growth and development of sclerotia of the fungus isolated from winter rapeseed and white mustard plants growing in the Western Ciscaucusia to develop the most effective measures to protect these crops from the disease. The experiments were conducted in laboratory conditions in 2024. The sclerotia of the fungus , collected in 2023 from the affected stems of the white mustard variety Raduga (Labinsky district) and in 2024 from the affected stems of the winter rapeseed variety Loris (Krasnodar), were the object of this study. The laboratory study included four variants, each of which was examined using 10 sclerotia: a nutrient semi-synthetic medium PGA (potato-glucose agar) at an air temperature of 25 °C; a nutrient semi-synthetic medium PGA at an air temperature of 27 °C; a Czapeks nutrient synthetic medium at an air temperature of 25 °C; and a Czapeks nutrient synthetic medium at an air temperature of 27 °C. Studies of the biological properties of under laboratory conditions are preferably carried out using the PGA nutrient medium. The rate of pathogen sclerotia germination on PGA is higher than the rate of mycelium growth on Czapeks agar: 1.5 times on average at 25 °C and 3.6 times on average at 27 °C. Using Czapeks agar to cultivate isolates slows the formation of new fungal sclerotia and reduces their number by 28–35 %.

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Cite This Study

O.A. Serdyuk (2025) studied this question.

synapsesocial.com/papers/698d6de45be6419ac0d5327chttps://doi.org/10.7868/s3034582025040083
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