Decoupling Geometric and Topological Contributions to Conformational Free-Energy Landscapes in DNA Origami Nanosheets

Key Points

• The research aims to quantify the independent contributions of geometric and topological factors to the mechanics of DNA origami nanosheets.
• Analyzed the intrinsic twist and scaffold topology effects on DNA origami mechanics.
• Conducted experiments to measure bending responses in various configurations.
• Developed models to separate geometric and topological influences on conformational energy landscapes.
• Identified distinct contributions of intrinsic twist and topology to the bending behavior of DNA origami.
• Demonstrated that geometric and topological factors interact to complicate bending predictions.
• Provided quantitative measurements that outline how these factors influence conformational free-energy.